UPCOMING: Hands-on PCR training!
This Saturday Dec 7th, DIYBio.to welcomes you to an offsite hands-on PCR workshop! No experience necessary. This is meant to be a fun and informative exploration of PCR, which is the cornerstore tech of molecular biology. REGISTRATION REQUIRED in advance, as materials must be prepared in advance. Please register here:
https://www.meetup.com/diybio-toronto/events/304848468/?slug=diybio-toronto&eventId=304848468
Course Description: Basic PCR protocol for Standard PCR and some Real-time qPCR. Learn to micropipette and set up PCR reactions.
Expectations and goals: Learn what PCR is and how to perform it.
DNA amplification using short DNA oligonucleotides, an enzyme to copy the DNA strands, and amplify them by attaching the nucleotides that correspond to the opposite strand. Given a set of primers that is well defined for the specific target, the method is fairly straightforward on paper, but requires attention to detail in practice. Using a Taq mix, the primers and mix are added together. Making Taq mixes from scratch is also possible, and requires utmost care in the preparation – keeping all DNA samples away from the preparation site. The next step is adding the template or test sample, and setting up the PCR machine. Afterwards, we will also run a gel.
Past classes:
Previous Eventbrite classes in PCR:
https://www.eventbrite.ca/e/911671673517?aff=oddtdtcreator